siRNA on ImagineArray™ Beachfront

Beachfront consists of 2x200 spots arranged on two arrays (width: 9 spots, height: 32 spots) placed at opposite ends of the plate. Each array holds:

  • 40 spots of siRNA targetting RELA,
  • 32 spots targetting INCENP,
  • 128 control spots of non-targetting siRNA.

Each cellular component is easily identified through colored labelling. RNA spots are labeled with red siGlo, nuclei with blue hoechst and the RELA/p65 protein with GFB bound antibodies dye. The control spot shows an even distribution of cytoplasmic p65 with a single nuclei in the center of cells. RELA spots show the absence of cytoplasmic green staining in cells indicating that RELA has been silenced. INCENP spots show cells with multiple or enlarged nuclei.

Clickable layout with experiment images below

  • rela_collage_slide.png
    RELA spot
  • incenp_collage_slide.png
    INCENP spot
  • control_collage_slide.png
    Control spot
image/svg+xml INCENP CONTROL 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 I H G F E D C B A 1 2 3 4 5 6 7 8