RELA silencing via reverse transfection of miRNA

We performed a screen to identify miRNA that affect RELA expression using the Persomics platform. The miRNA arrays were covered with HeLa cells for 48h to allow miRNA transfection. Cells were then fixed and stained, detecting RELA by indirect immuno-labelling with an anti-RELA primary and a green fluorescent secondary.

One of the miRNA we identified was HSA MIR 373 3P which is a known regulator of RELA expression. Below are 3 replicates of control spots (non-targeting siRNA) and spots with HSA MIR 373 3P respectively.

Control 1 - spot  Control 1 - cells Control 1 - full image 
 miRNA 1 - spot miRNA 1 - cells miRNA 1 - full image
Control 2 - spot Control 2 - cells Control 2 - full image
 miRNA 2 - spot miRNA 2 - cells miRNA 2 - full image
 Control 3 - spot Control 3 - cells Control 3 - full image
 miRNA 3 - spot miRNA 3 - cells miRNA 3 - full image