The following protocol is followed when screening at Persomics labs. Note that this protocol should be adapted to the conditions of your assay and specific cell lines.
Disclaimer: The publication of this protocol template does not constitute a guarantee on the part of Persomics and its affiliates shall not be liable for the results in individual experiments.
Always adhere to the safety protocols prescribed by your lab.
Primary Antibody: Rabbit IgG 1:2000 in 10% goat serum diluted with PBS
Secondary Antibody: Fluorescent Anti-Rabbit IgG 1:1000 in 10% goat serum diluted with PBS
Hoechst: 10mM Hoechst 33342 in DMSO diluted 1:1000 in PBS
Fix: [4.0% w/v Paraformaldehyde in PBS pH 7.4]
Perm: [0.1% v/v TritonX-100 in PBS]
Blocking Buffer/Goat serum: [10% v/v goat serum in PBS]
Follow basic tissue culture protocol:
Use appropriate trypsin, media, and incubation parameters for specific cell line.
Use appropriate PBS for specific cell line. Aspirate and pipette on corner of plate. DO NOT TOUCH GLASS!